pKSM714 (ATCC® 87160)

Applications: expression vectorvector permitting RNA synthesis in vitrovector permitting production of single-stranded DNAvector permitting visual detection of recombinants  /  Depositors: K Ippen-Ihler, S Maneewannakul

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Designations pKSM714
Depositors K Ippen-Ihler, S Maneewannakul
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Distribution host: Escherichia coli DH5alpha
Vector Information
Size (kb): 5.0409998893737790
Intact vector size: 5.041
Type of vector: phagemid
Cloning sites: BamHI HindIII XbaI AvaI SmaI EcoRI
Polylinker sites: BamHI HindIII XbaI AvaI SmaI EcoRI
Construction: pKSM710, pSM102
Host range: Escherichia coli
Features (with orientation and position when available):
promoter for in vitro transcription: T7lac, ->, 20-36
promoter for expression: lacUV5, ->, 197-226
MCS: BamHI...EcoRI, ->, 340-391
insert detection: lacZalpha, ->, 335-607
transcription terminator: T7, ->, 609-650
transcription terminator: rrnB, ->, 707-750
transcription terminator: rrnB, ->, 881-910
transcription terminator: fd, ->, 1006-1052
transcription terminator: fd, ->, 1116-1162
replicon: fd, ->, 1295-1750
replicon: pMB1, <-, 1903-2497
repressor gene: lacIq, ->, 2666-3748
marker(s): kanR, ->, 4174-4968
terminator: trpA, ->, 4989-5016
Vector: pKSM714 (phagemid)
Promoters: Promoter for in vitro transcription T7lac
Construction: pKSM710, pSM102
Construct size (kb): 5.040999889373779
Features: insert detection: lacZalpha
marker(s): kanR
promoter for expression: lacUV5
promoter for in vitro transcription: T7lac
replicon: fd
replicon: pMB1
repressor gene: lacIq
MCS: BamHI...EcoRI
terminator: trpA
transcription terminator: T7
transcription terminator: fd
transcription terminator: rrnB
expression vector
vector permitting RNA synthesis in vitro
vector permitting production of single-stranded DNA
vector permitting visual detection of recombinants
Restriction digests of the clone give the following sizes (kb): BamHI--5.0; EcoRI--5.0; PstI--4.2, 0.65.
Vector contains the following restriction sites (approximate kb from nt 1): BamHI--0.34; BglII--4.14; EcoRI--0.39; KpnI--0.36, 2.59; NcoI--0.33, 4.74; PstI--4.35, 5.03; PvuI--0.51; PvuII--0.48, 3.62, 3.71, 4.05, 4.41; SacI--0.38, 2.58; SalI--5.04.
Differs from pKSM713 (ATCC 87159) only in the orientation of the pSM102 fragment containing lacIq, kanR and the trpA terminator.
Expression vector.
Media ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 37.0°C

Maneewannakul S, et al. The pKSM710 vector cassette provides tightly regulated lac and T7lac promoters and strategies for manipulating N-terminal protein sequences. Plasmid 31: 300-307, 1994. PubMed: 8058823

Shipped freeze-dried
Shipping Information Distributed: freeze-dried