pKSM714 (ATCC® 87160)

Applications: expression vectorvector permitting RNA synthesis in vitrovector permitting production of single-stranded DNAvector permitting visual detection of recombinants  /  Depositors: K Ippen-Ihler, S Maneewannakul

Designations pKSM714
Permits and Restrictions

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Depositors K Ippen-Ihler, S Maneewannakul
Biosafety Level 1
Distribution host: Escherichia coli DH5alpha
Vector Information
Size (kb): 5.0409998893737790
Intact vector size: 5.041
Type of vector: phagemid
Cloning sites: BamHI HindIII XbaI AvaI SmaI EcoRI
Polylinker sites: BamHI HindIII XbaI AvaI SmaI EcoRI
Construction: pKSM710, pSM102
Host range: Escherichia coli
Features (with orientation and position when available):
promoter for in vitro transcription: T7lac, ->, 20-36
promoter for expression: lacUV5, ->, 197-226
MCS: BamHI...EcoRI, ->, 340-391
insert detection: lacZalpha, ->, 335-607
transcription terminator: T7, ->, 609-650
transcription terminator: rrnB, ->, 707-750
transcription terminator: rrnB, ->, 881-910
transcription terminator: fd, ->, 1006-1052
transcription terminator: fd, ->, 1116-1162
replicon: fd, ->, 1295-1750
replicon: pMB1, <-, 1903-2497
repressor gene: lacIq, ->, 2666-3748
marker(s): kanR, ->, 4174-4968
terminator: trpA, ->, 4989-5016
Vector: pKSM714 (phagemid)
Promoters: Promoter for in vitro transcription T7lac
Construction: pKSM710, pSM102
Construct size (kb): 5.040999889373779
Features: insert detection: lacZalpha
marker(s): kanR
promoter for expression: lacUV5
promoter for in vitro transcription: T7lac
replicon: fd
replicon: pMB1
repressor gene: lacIq
MCS: BamHI...EcoRI
terminator: trpA
transcription terminator: T7
transcription terminator: fd
transcription terminator: rrnB
expression vector
vector permitting RNA synthesis in vitro
vector permitting production of single-stranded DNA
vector permitting visual detection of recombinants
Restriction digests of the clone give the following sizes (kb): BamHI--5.0; EcoRI--5.0; PstI--4.2, 0.65.
Vector contains the following restriction sites (approximate kb from nt 1): BamHI--0.34; BglII--4.14; EcoRI--0.39; KpnI--0.36, 2.59; NcoI--0.33, 4.74; PstI--4.35, 5.03; PvuI--0.51; PvuII--0.48, 3.62, 3.71, 4.05, 4.41; SacI--0.38, 2.58; SalI--5.04.
Differs from pKSM713 (ATCC 87159) only in the orientation of the pSM102 fragment containing lacIq, kanR and the trpA terminator.
Expression vector.
Media ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 37.0°C

Maneewannakul S, et al. The pKSM710 vector cassette provides tightly regulated lac and T7lac promoters and strategies for manipulating N-terminal protein sequences. Plasmid 31: 300-307, 1994. PubMed: 8058823

Shipped freeze-dried
Shipping Information Distributed: freeze-dried