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Vector Information
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Size (kb): 4.3000001907348630 Vector: pBSL97 (phagemid) Promoters: Promoter for in vitro transcription T7 (phi10) Marker(s):ampR,kanR Construct size (kb): 4.300000190734863 Features: marker(s): ampR
promoter for in vitro transcription: T7 (phi10)
replicon: f1
replicon: pMB1
MCS: KpnI...SacI MluI
MCS: MluI SacI...KpnI
coding sequence: nptII (kanR)
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Applications
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contains easily purifiable cassette(s) for construction aminoglycoside phosphotransferase kanamycin resistance, neomycin resistance
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Comments
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Restriction digests of the clone give the following sizes (kb): BamHI--2.6, 1.3; EcoRI--2.75, 1.4; KpnI--2.5, 1.45; MluI--2.85, 1.25; SstI--2.85, 1.25. Plasmid useful for vector construction and generation of marked deletions and insertions in cloned DNA. SphI and PstI sites in the nptII gene were removed by site-directed mutagenesis. Contains a nptII (kanR) cassette flanked by multiple cloning sites duplicated in inverted orientation. pBSL97 ( ATCC 87140) and pBSL99 ( ATCC 87141) differ in the orientations of the multiple cloning sites. |
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References
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Alexeyev MF, et al. Improved antibiotic-resistance gene cassettes and omega elements for Escherichia coli vector contruction and in vitro deletion/insertion mutagenesis. Gene 160: 63-67, 1995. PubMed: 7628718
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