pBend5 (ATCC® 87123)

Applications: vector containing primer sites useful for sequencingvector for studying DNA-protein interactions  /  Depositors: C Zwieb

Permits and Restrictions

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Designations pBend5
Depositors C Zwieb
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 3.1960000991821290
Vector: pBend5 (plasmid)
Promoters: Promoter T7 (phi10)
Construction: pBluescriptSK-
Construct size (kb): 3.196000099182129
Features: marker(s): ampR
promoter: T3
promoter: T7 (phi10)
replicon: pMB1
MCS: HindIII...EcoRI
vector containing primer sites useful for sequencing
vector for studying DNA-protein interactions
Restriction digests of the clone give the following sizes (kb): BglI--1.6, 1.25, 0.30, 0.25; EcoRI/HindIII--2.9, 0.29; XbaI--3.2.
Recommendation for verification: EcoRI+HindIII--2.95, 0.25; BglI--1.9, 1.3; XbaI--3.2.
Target DNA binding sites can be cloned into the vector and an array of fragments can be generated (by restriction digestion with different enzymes) that are equal in length, but differ in the position of the DNA binding site along the fragment.
The reduced mobility of different protein-fragment complexes can then by analyzed by gel electrophoresis to determine the degree of bending induced by protein binding.
One of three vectors (ATCC 87121-87123) designed for studying the amount of bending induced at a DNA binding site due to DNA-protein interactions. The three vectors differ only in the available cloning sites.
Vector constructed from pBend4 (ATCC 87122) by destruction of one of the SalI sites in the polylinker.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Kim J, et al. Bending of DNA by gene-regulatory proteins: construction and use of a DNA bending vector. Gene 85: 15-23, 1989. PubMed: 2533576

Zwieb C, Adhya SImproved plasmid vectors for the analysis of protein-induced DNA bendingIn: Zwieb C, Adhya SMethods in Molecular Biology, Vol 30: DNA-Protein interactions: Principles and ProtocolsTotowa, NJThe Humana Press, Inc.pp. 281-294, 1994

Shipped freeze-dried
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