pJC20 plasmid in E. coli (ATCC® 87113)

Applications: expression vectorvector for generating nested deletions of cloned inserts  /  Depositors: J Clos

Designations pJC20 plasmid in E. coli
Permits and Restrictions

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Depositors J Clos
Biosafety Level 1
Vector Information
Size (kb): 2.3399999141693120
Vector: pJC20 plasmid in E. coli (plasmid)
Promoters: Promoter for expression T7 (phi10)
Construction: pJC10
Construct size (kb): 2.339999914169312
Features: marker(s): ampR
promoter for expression: T7 (phi10)
replicon: pMB1
MCS: NdeI...ApaI
ribosome-binding site: Shine-Dalgarno
terminator: T phi
expression vector
vector for generating nested deletions of cloned inserts
Restriction digests of the clone give the following sizes (kb): EcoRI--2.3; HindIII--2.3; XbaI--2.3.
High-copy-number expression vector (T7-based) that can be used for preparation of nested deletions.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Clos J, Brandau S. pJC20 and pJC40--two high-copy-number vectors for T7 RNA polymerase-dependent expression of recombinant genes in Escherichia coli. Protein Expr. Purif. 5: 133-137, 1994. PubMed: 8054844

Clos J, et al. Molecular cloning and expression of hexameric Drosophila heat shock factor subject to negative regulation. Cell 63: 1085-1097, 1990. PubMed: 2257625

Shipped freeze-dried
Shipping Information

 Freeze dried plasmid in E. coli