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Vector Information
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Size (kb): 5.4000000953674320 Vector: YCpIF11 (plasmid) Promoters: Promoter for expression GAL1 Construction: pUN15 Marker(s):TRP1,ampR Features: marker(s): TRP1
marker(s): ampR
promoter for expression: GAL1
replicon: ARS1
replicon: pMB1
MCS: SacI...SalI
centromere: CEN4
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Applications
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YX-type (expression) shuttle vector
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Comments
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Restriction digests of the clone give the following sizes (kb): XbaI--3.6, 1.8; BamHI--5.4; EcoRI--5.4. One of several S. cerevisiae shuttle expression vectors (ATCC 87078-87092) differing in selection marker, reading frame and orientation of the promoter. Vectors YCpIF10, YCpIF11 and YCpIF12 (ATCC 87087-87089) differ only in the reading frame of the multiple cloning site.
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References
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Foreman PK, Davis RW. Cloning vectors for the synthesis of epitope-tagged, truncated and chimeric proteins in Saccharomyces cerevisiae. Gene 144: 63-68, 1994. PubMed: 7517907
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