Nucleotide (GenBank) :
Expression cloning vector pT7/Nco, complete sequence.
Size (kb): 2.9000000953674320
Vector: pT7/Nde (plasmid)
Promoters: Promoter SP6
Construction: pGEM-3, pET-3a
Construct size (kb): 2.900000095367432
Features: marker(s): ampR
promoter: T7 (phi10)
ribosome-binding site: gene 10
Restriction digests of the clone give the following sizes (kb): XbaI--2.90; HindIII--2.90; NdeI--2.90.
The NdeI site (CATATG) includes the initiator methionine codon. The NdeI cloning site can also be used in conjunction with the downstream BamHI site.
Differs from pT7/NcoI (ATCC 87051)
only in the cloning site and reading frame for the insert.
Expression vector, maintained at high copy number.
Hong JX, et al. Effect of ADP-ribosylation factor amino-terminal deletions on its GTP-dependent stimulation of cholera toxin activity. J. Biol. Chem. 269: 9743-9745, 1994. PubMed: 8144566