pAS2 [pAS1-CYH2] (ATCC® 87008)

Applications: YE-type (episomal) shuttle vectorcontains sequence ribosomal protein L29 Ribosomal protein RL43expression vectorshuttle vectorvector for constructing cDNA librariesvector for other uses  /  Depositors: SJ Elledge

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Designations pAS2 [pAS1-CYH2]
Depositors SJ Elledge
Biosafety Level 1
Vector Information
Size (kb): 8.5000000000000000
Vector: pAS2 (phagemid)
Promoters: Promoter ADC1 (ADH)
Construction: pAS1
Marker(s):TRP1,ampR,cyhS
Features: marker(s): CYH2
marker(s): TRP1
marker(s): ampR
other: GAL4 DNA-binding domain
promoter: ADC1 (ADH)
replicon: 2 micron
replicon: f1+
replicon: pMB1
MCS: NdeI...SalI
terminator: ADC1
epitope tag: hemagglutinin (HA)
Applications
YE-type (episomal) shuttle vector
contains sequence ribosomal protein L29 Ribosomal protein RL43
expression vector
shuttle vector
vector for constructing cDNA libraries
vector for other uses
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--8.6; SalI--8.6; SmaI--8.6.
Shuttle expression vector used to create fusion proteins consisting of the nuclear localization sequence from SV40 T antigen, the GAL4 DNA-binding domain (aa 1-147), and a HA (hemagglutinin) epitope tag in frame with the activation domain.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Harper JW, et al. The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases. Cell 75: 805-816, 1993. PubMed: 8242751

Shipped freeze-dried
Shipping Information

 Freeze dried E. coli containing the plasmid

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