pACT2 [pACTII] (ATCC® 87006)

Applications: YE-type (episomal) shuttle vectorexpression vectorvector component of two-hybrid screening systemvector for constructing cDNA libraries  /  Depositors: SJ Elledge

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Designations pACT2 [pACTII]
Depositors SJ Elledge
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Host
Distribution host: Escherichia coli
Vector Information
Size (kb): 7.5500001907348630
DESCRIPTION OF VECTOR:
Intact vector size: 7.550
Type of vector: plasmid
Cloning sites: NcoI SfiI SmaI BamHI EcoRI XhoI BglII
Polylinker sites: NdeI NcoI SfiI SmaI BamHI EcoRI XhoI BglII
Construction: pSE1107 (pACT1)
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
promoter: ADC1 (ADH), ->
other: GAL4 activation domain, ->
epitope tag: hemagglutinin (HA), ->
MCS: BglII/NdeI...BglII, ->
terminator: ADC1
marker(s): LEU2, <-
replicon: 2 micron
marker(s): ampR
replicon: pMB1
Vector: pACT2 (plasmid)
Promoters: Promoter ADC1 (ADH)
Construction: pSE1107 (pACT1)
Marker(s):LEU2,ampR
Construct size (kb): 7.550000190734863
Features: marker(s): LEU2
marker(s): ampR
other: GAL4 activation domain
promoter: ADC1 (ADH)
replicon: 2 micron
replicon: pMB1
MCS: BglII/NdeI...BglII
terminator: ADC1
epitope tag: hemagglutinin (HA)
Applications
YE-type (episomal) shuttle vector
expression vector
vector component of two-hybrid screening system
vector for constructing cDNA libraries
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--7.6; BamHI--7.6; BglII--7.5.
Shuttle expression vector used to create fusion proteins consisting of the nuclear localization sequence from SV40 T antigen, the GAL4 activation domain (aa 768-881) and a HA (hemagglutinin) epitope tag in frame with the activation domain.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Stephen J Elledge, personal communication

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component of:ATCC 87014
Shipped freeze-dried
Shipping Information Distributed: freeze-dried