pACT2 [pACTII] (ATCC® 87006)

Applications: YE-type (episomal) shuttle vectorexpression vectorvector component of two-hybrid screening systemvector for constructing cDNA libraries  /  Depositors: SJ Elledge

Designations pACT2 [pACTII]
Permits and Restrictions

View Permits

Depositors SJ Elledge
Biosafety Level 1
Distribution host: Escherichia coli
Vector Information
Size (kb): 7.5500001907348630
Intact vector size: 7.550
Type of vector: plasmid
Cloning sites: NcoI SfiI SmaI BamHI EcoRI XhoI BglII
Polylinker sites: NdeI NcoI SfiI SmaI BamHI EcoRI XhoI BglII
Construction: pSE1107 (pACT1)
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
promoter: ADC1 (ADH), ->
other: GAL4 activation domain, ->
epitope tag: hemagglutinin (HA), ->
MCS: BglII/NdeI...BglII, ->
terminator: ADC1
marker(s): LEU2, <-
replicon: 2 micron
marker(s): ampR
replicon: pMB1
Vector: pACT2 (plasmid)
Promoters: Promoter ADC1 (ADH)
Construction: pSE1107 (pACT1)
Construct size (kb): 7.550000190734863
Features: marker(s): LEU2
marker(s): ampR
other: GAL4 activation domain
promoter: ADC1 (ADH)
replicon: 2 micron
replicon: pMB1
MCS: BglII/NdeI...BglII
terminator: ADC1
epitope tag: hemagglutinin (HA)
YE-type (episomal) shuttle vector
expression vector
vector component of two-hybrid screening system
vector for constructing cDNA libraries
Restriction digests of the clone give the following sizes (kb): EcoRI--7.6; BamHI--7.6; BglII--7.5.
Shuttle expression vector used to create fusion proteins consisting of the nuclear localization sequence from SV40 T antigen, the GAL4 activation domain (aa 768-881) and a HA (hemagglutinin) epitope tag in frame with the activation domain.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Stephen J Elledge, personal communication

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