pXC33b (ATCC® 86995)

Applications: expression vectorin another host, produces proteinvector useful for cloning PCR products  /  Depositors: TA Patterson

Designations pXC33b
Permits and Restrictions

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Depositors TA Patterson
Biosafety Level 1
Vector Information
Size (kb): 5.7760000228881840
Vector: pXC33b (plasmid)
Promoters: Promoter for expression lambda PL
Construction: pXC24, oligo cassette
Construct size (kb): 5.776000022888184
Features: initiation codon: ATG
marker(s): ampR
marker(s): tetR
promoter for expression: lambda PL
replicon: ROP copy number control
replicon: pMB1
restriction site: 3' sites HindIII...BamHI
restriction site: 5' PacI site
ribosome-binding site: synthetic Shine-Dalgarno
spacer sequence: ATTAATTA
transcription terminator: partially deleted lambda terminator
translational enhancer: T7 gene 10
coding sequence: 14-3-3
expression vector
in another host, produces protein
vector useful for cloning PCR products
Restriction digests of the clone give the following sizes (kb): BamHI--5.8; EcoRI--5.8; HindIII--5.8.
The vector contains a 0.7 kb bovine cDNA that can be excised using the 5' and 3' cloning sites and be replaced by a gene of interest. The bovine insert may be used as a positive control for expression.
One of 12 expression vectors (ATCC 86990-87001) designed to maximize expression from the lambda PL promoter and support cloning of PCR products. The vectors differ in cloning sites and in translational enhancer and initiation sequences.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Cheng X, Patterson TA. Construction and use of lambda PL promoter vectors for direct cloning and high level expression of PCR amplified DNA coding sequences. Nucleic Acids Res. 20: 4591-4598, 1992. PubMed: 1408761

Shipped freeze-dried
Shipping Information

Shipped: Freeze dried Escherichia coli TAP308 containing the plasmid.