pJCF62 (ATCC® 86982)

Applications: contains sequence DNA Segmentshuttle vector  /  Depositors: JG Cobley

Designations pJCF62
Permits and Restrictions

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Depositors JG Cobley
Biosafety Level 1
Vector Information
Size (kb): 12.1000003814697300
Vector: pJCF62 (plasmid)
Construction: pJCF22, pFDA ori
Construct size (kb): 12.10000038146973
Features: marker(s): kanR, cmlR
replicon: pMB1, pFDA
contains sequence DNA Segment
shuttle vector
Restriction digests of the clone give the following sizes (kb): HindIII--2.55, 2.4, 2.2 (doublet), 2.15, 1.1; BamHI--uncut; ClaI--12.1, ClaI/BamHI--12.1.
Can be mobilized into F. diplosiphon using the conjugative plasmid RP4.
Shuttle vector.
A 2.9 kb BamHI/ClaI fragment from a different construct containing the origin of replication has been sequenced. The BamHI site is not retained in this plasmid.
Constructed by inserting a 8.0 kb ClaI fragment, containing the F. diplosiphon pFDA origin of replication, into the AsuII site of pJCF22.
The order of the major features of the plasmid is: kanR - cmlR - F. diplosiphon pFDA ori - bom - oriV.
Media ATCC® Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol
Growth Conditions
Temperature: 37.0°C

Schaefer MR, et al. Plasmids from two morphologically distinct cyanobacterial strains share a novel replication origin. J. Bacteriol. 175: 5701-5705, 1993. PubMed: 8366056

Cobley JG, et al. Construction of shuttle plasmids which can be efficiently mobilized from Escherichia coli into the chromatically adapting cyanobacterium Fremyella diplosiphon. Plasmid 30: 90-105, 1993. PubMed: 8234495

John G Cobley, personal communication

Shipped freeze-dried