Size (kb): 12.1000003814697300
Vector: pJCF31 (plasmid)
Construction: pJCF22, pFDA ori
Construct size (kb): 12.10000038146973
Features: marker(s): cmlR
replicon: pMB1, pFDA
contains sequence DNA Segment
Restriction digests of the clone give the following sizes (kb): ClaI--8.0, 4.1; BamHI--uncut; HindIII--3.9, 2.3, 2.1, 1.9, 1.0, 0.9.
Can be mobilized into F. diplosiphon using the conjugative plasmid RP4.
A 2.9 kb BamHI/ClaI fragment from a different construct containing the origin of replication has been sequenced. The BamHI site is not retained in this plasmid.
Constructed by inserting a 8.0 kb ClaI fragment, containing the F. diplosiphon pFDA origin of replication, into the ClaI site of pJCF22.
The order of the major features of the plasmid is: cmlR - bom - oriV - F. diplosiphon pFDA ori.
Schaefer MR, et al. Plasmids from two morphologically distinct cyanobacterial strains share a novel replication origin. J. Bacteriol. 175: 5701-5705, 1993. PubMed: 8366056
Cobley JG, et al. Construction of shuttle plasmids which can be efficiently mobilized from Escherichia coli into the chromatically adapting cyanobacterium Fremyella diplosiphon. Plasmid 30: 90-105, 1993. PubMed: 8234495
John G Cobley, personal communication