pJCF31 [pJCF22.H51] (ATCC® 86981)

Applications: contains sequence DNA Segmentshuttle vector  /  Depositors: JG Cobley

Designations pJCF31 [pJCF22.H51]
Depositors JG Cobley
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 12.1000003814697300
Vector: pJCF31 (plasmid)
Construction: pJCF22, pFDA ori
Construct size (kb): 12.10000038146973
Features: marker(s): cmlR
replicon: pMB1, pFDA
contains sequence DNA Segment
shuttle vector
Restriction digests of the clone give the following sizes (kb): ClaI--8.0, 4.1; BamHI--uncut; HindIII--3.9, 2.3, 2.1, 1.9, 1.0, 0.9.
Can be mobilized into F. diplosiphon using the conjugative plasmid RP4.
Shuttle vector.
A 2.9 kb BamHI/ClaI fragment from a different construct containing the origin of replication has been sequenced. The BamHI site is not retained in this plasmid.
Constructed by inserting a 8.0 kb ClaI fragment, containing the F. diplosiphon pFDA origin of replication, into the ClaI site of pJCF22.
The order of the major features of the plasmid is: cmlR - bom - oriV - F. diplosiphon pFDA ori.
Media ATCC® Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol
Growth Conditions
Temperature: 37.0°C

Schaefer MR, et al. Plasmids from two morphologically distinct cyanobacterial strains share a novel replication origin. J. Bacteriol. 175: 5701-5705, 1993. PubMed: 8366056

Cobley JG, et al. Construction of shuttle plasmids which can be efficiently mobilized from Escherichia coli into the chromatically adapting cyanobacterium Fremyella diplosiphon. Plasmid 30: 90-105, 1993. PubMed: 8234495

John G Cobley, personal communication

Shipped freeze-dried