lambdaSK20 [clone 3] (ATCC® 86970)

Applications: vector containing primer sites useful for sequencingvector for constructing genomic librariesvector for other usesvector permitting positive selection for inserts  /  Depositors: ER Zabarovsky, G Winberg, G Klein

Designations lambdaSK20 [clone 3]
Permits and Restrictions

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Depositors ER Zabarovsky, G Winberg, G Klein
Biosafety Level 1
Vector Information
Size (kb): 47.7999992370605500
Vector: lambdaSK20 (phage, lambda - replacement)
Marker(s):red+ gam+
Construct size (kb): 47.79999923706055
Features: insert detection: Spi+
marker(s): red+ gam+
replicon: lambda, pMB1, M13
vector containing primer sites useful for sequencing
vector for constructing genomic libraries
vector for other uses
vector permitting positive selection for inserts
Restriction digests of the clone give the following sizes (kb): BamHI--21.0, 13.5 (doublet); EcoRI--21.0, 13.5 (doublet); SalI--20.3, 12.0, 9.4, 6.0.
Vector permitting positive selection (Spi) of recombinants. Useful for conversion to phagemid (without selection). Permits construction using partial filling-in.
The restriction sites to be used for conversion to phagemid are: SalI SacII BglI SfiI NotI XmaIII.
Cloning capacity: 18.9 kb.
The sizes of the major components are (kb): arms--20.3, 9.2; stuffer fragment--13.7; phagemid segments--0.7 (longer arm), 3.9.
The order of the major features in this plasmid is: arm - SalI/XmaIII - XbaI/MCS/NaeI - stuffer - NaeI/MCS/SacI - M13 ori - ampR - pMB1 ori - XmaIII/SalI - arm.

Zabarovsky ER, et al. Lambda SK diphasmids: phage lambda vectors for genomic, jumping, linking and cDNA libraries. Gene 127: 1-14, 1993. PubMed: 8486275

Zabarovsky ER, Winberg GN. Improved methods and new vectors for creating libraries of cloned genes. Sov. Sci. Rev. 11: 1-65, 1992.

Shipped freeze-dried