pJK142 (ATCC® 86955)

Applications: expression vectorvector permitting visual detection of recombinants  /  Depositors: JB Keeney

Permits and Restrictions

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Designations pJK142
Depositors JB Keeney
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 3.2720000743865970
Vector: pJK142 (plasmid)
Promoters: Promoter lac
Construct size (kb): 3.272000074386597
Features: insert detection: lac
marker(s): ampR
promoter: lac
replicon: pMB1
expression vector
vector permitting visual detection of recombinants
Restriction digests of the clone give the following sizes (kb): BamHI--3.3; EcoRI--3.3; HindIII--3.3.
Expression vector permitting visual detection of recombinants by lacZalpha complementation.
The order of the major features in the plasmid is: ampR - pMB1 ori - 5' lacZ' - SacI/MCS/KpnI - 3' lacZ' - NdeI.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Keeney JB, Boeke JD. Efficient targeted integration at leu1-32 and ura4-294 in Schizosaccharomyces pombe. Genetics 136: 849-856, 1994. PubMed: 8005439

Jill B Keeney, personal communication

Shipped freeze-dried