Size (kb): 7.0000000000000000
Vector: pUK4134 (plasmid)
Construction: pRTP1, pJM703.1
Construct size (kb): 6.699999809265137
Features: marker(s): ampR
other: lambda cos site
contains sequence origin of transfer replication
contains sequence ribosomal protein S12
vector permitting positive selection for integration
Restriction digests of the clone give the following sizes (kb): BamHI--6.7, 0.5; EcoRI--6.4, 0.8; EcoRV--7.2; SalI--7.2.
RP6 is a suicide replicon in hosts not producing the pir protein.
RpsL (streptomycin sensitive) is dominant to streptomycin resistance, so loss of the plasmid can identified by streptomycin resistance and ampicillin sensitivity.
Suicide, gene replacement vector for Yersiniae and other gram-negative hosts that permits positive selection for loss of the plasmid after allelic exchange.
The vector also contains cos sites and oriT, and can be used for cosmid cloning or conjugative transfer.
The order of the major features in the plasmid is: EcoRV - R6K ori - oriT - SalI - cos - rpsL - ampR.
Skrzypek E, et al. New suicide vector for gene replacement in Yersiniae and other gram-negative bacteria. Plasmid 29: 160-163, 1993. PubMed: 8469722
Miller VL, Mekalanos JJ. A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR. J. Bacteriol. 170: 2575-2583, 1988. PubMed: 2836362