pI3 (ATCC® 77461)

Applications: shuttle vector  /  Depositors: KW Minton, C Masters

Designations pI3
Permits and Restrictions

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Depositors KW Minton, C Masters
Biosafety Level 1
Vector Information
Size (kb): 16.0000000000000000
Vector: pI3 (plasmid)
Construction: pKK232-8, pUE10
Construct size (kb): 16.0
Features: marker(s): ampR, cmlR
replicon: pMB1, pUE10
shuttle vector
Restriction digests of the clone give the following sizes (kb): BamHI--17.0; EcoRI--14.0, 4.3; HindIII--17.0.
Shuttle vector using ampR selection in E. coli and cmlR selection in Deinococcus radiodurans.
The order of the major features in this plasmid is: EcoRI/MCS/HindIII - pUE10 replicon - pUE10 promoter -> - cmlR -> - pMB1 ori - ampR.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Masters CI, Minton KW. Promoter probe and shuttle plasmids for Deinococcus radiodurans. Plasmid 28: 258-261, 1992. PubMed: 1461940

Shipped freeze-dried