pGPTV-KAN (ATCC® 77388)

Applications: shuttle vector  /  Depositors: R Masterson, D Becker

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Designations pGPTV-KAN
Depositors R Masterson, D Becker
Biosafety Level 1
Host
Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 13.5000000000000000
DESCRIPTION OF VECTOR:
Intact vector size: 13.500
Type of vector: plasmid
Cloning sites: EcoRI SstI SmaI XbaI SalI HindIII BamHI
Polylinker sites:
Construction: pBIN19, pBI101, pBIB-HYG
Host range: Rhizobium radiobacter; higher plants; Escherichia coli
Features (with orientation and position when available):
marker(s): kanR
replicon: RK2
Vector: pGPTV-KAN (plasmid)
Construction: pBIN19, pBI101, pBIB-HYG
Marker(s):kanR
Construct size (kb): 13.5
Features: marker(s): kanR
replicon: RK2
Applications
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--13.6; BglII--8.2, 3.2, 2.1; HindIII--13.6.
Vector contains the following restriction sites (approximate kb from the HindIII site): BamHI--1.4; EcoRI--11.4; SstI--11.7.
Binary vector with plant selectable marker near the T-DNA left border and a glucuronidase (uidA) reporter gene. The marker and uidA are divergently transcribed, and can be easily removed or replaced.
The order of the major features of this plasmid is: EcoRI - pAnos - SstI - uidA - SmaI - XbaI - SalI - HindIII - Pnos - BglII - nptII - BamHI - pAg7 - T-DNA - BglII - RK2 ori - kanR.
Media ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 37.0°C
References

Becker D, et al. New plant binary vectors with selectable markers located proximal to the left T-DNA border. Plant Mol. Biol. 20: 1195-1197, 1992. PubMed: 1463855

Shipped frozen
Shipping Information Distributed: freeze-dried
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