pGPTV-KAN (ATCC® 77388)

Applications: shuttle vector  /  Depositors: R Masterson, D Becker

Permits and Restrictions

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Designations pGPTV-KAN
Depositors R Masterson, D Becker
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 13.5000000000000000
Intact vector size: 13.500
Type of vector: plasmid
Cloning sites: EcoRI SstI SmaI XbaI SalI HindIII BamHI
Polylinker sites:
Construction: pBIN19, pBI101, pBIB-HYG
Host range: Rhizobium radiobacter; higher plants; Escherichia coli
Features (with orientation and position when available):
marker(s): kanR
replicon: RK2
Vector: pGPTV-KAN (plasmid)
Construction: pBIN19, pBI101, pBIB-HYG
Construct size (kb): 13.5
Features: marker(s): kanR
replicon: RK2
shuttle vector
Restriction digests of the clone give the following sizes (kb): EcoRI--13.6; BglII--8.2, 3.2, 2.1; HindIII--13.6.
Vector contains the following restriction sites (approximate kb from the HindIII site): BamHI--1.4; EcoRI--11.4; SstI--11.7.
Binary vector with plant selectable marker near the T-DNA left border and a glucuronidase (uidA) reporter gene. The marker and uidA are divergently transcribed, and can be easily removed or replaced.
The order of the major features of this plasmid is: EcoRI - pAnos - SstI - uidA - SmaI - XbaI - SalI - HindIII - Pnos - BglII - nptII - BamHI - pAg7 - T-DNA - BglII - RK2 ori - kanR.
Media ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 37.0°C

Becker D, et al. New plant binary vectors with selectable markers located proximal to the left T-DNA border. Plant Mol. Biol. 20: 1195-1197, 1992. PubMed: 1463855

Shipped frozen
Shipping Information Distributed: freeze-dried