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Vector Information
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Size (kb): 5.8460001945495610 Vector: pTB (plasmid) Promoters: Promoter metallothionein Construction: pT ( ATCC 77384)Marker(s):ampR Construct size (kb): 5.846000194549561 Features: insert detection: lacZ'
insert detection: lacZ/amb5
marker(s): ampR
promoter: metallothionein
replicon: pMB1
MCS: EcoRI...HindIII
MCS: NruI...HindIII
restriction site: NotI
restriction site: NsiI
terminator: SV40
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Applications
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expression vector shuttle vector vector permitting construction of fusion proteins vector permitting visual detection of recombinants
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Comments
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Restriction digests of the clone give the following sizes (kb): PvuI--2.6, 1.8, 1.4; EcoRI--3.3, 2.5; HindIII--4.5, 1.3; BglII--5.8; ClaI--5.8. The plasmid contains the following restriction sites (bp from 0): NotI--0; EcoRI--9, 3272; SacI--1593; PvuI--1842, 3197, 4987; ClaI--1981; XhoI--2005; BglII--2028; SphI--3153, 3356; NsiI--3155; HindIII--2040, 3368; DrdI--3829, 5700; BsaI--4687. Expression vector containing two lacZalpha-based multiple cloning sites, with the second containing an amber mutation and being flanked by a mouse metallothionein I promoter at one end and splicing and polyadenylation signals at the other. The first site was designed for insertion of a marker selectable in mammalian cells, and the second for the sequence to be expressed. Both sites permit detection of inserts by alpha complementation, with discrimination between the two provided by the amber mutation in the second lacZ.
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References
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Wang Q, et al. Mammalian expression vectors with modulatable promoters and two multiple cloning sites. Gene 119: 155-161, 1992. PubMed: 1327962
J J McCormick, personal communication
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