pC4ro3 (ATCC® 77319)

Applications: contains sequence roughexpression vectorin another host, produces protein roughreporter constructshuttle vector  /  Depositors: TJ Lockett

Designations pC4ro3
Permits and Restrictions

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Depositors TJ Lockett
Biosafety Level 1
Vector Information
Size (kb): 10.8000001907348600
Vector: pC4ro3 (plasmid)
Construction: pro1, Carnegie 4, pSL1180 MCS
Construct size (kb): 10.80000019073486
Features: marker(s): ampR
replicon: pMB1
contains sequence rough
expression vector
in another host, produces protein rough
reporter construct
shuttle vector
Restriction digests of the clone give the following sizes (kb): SalI--8.6, 2.2; HindIII--7.7, 3.1; PstI--10.8.
The SphI/HindIII fragment corresponding to the rough gene contains the following restriction sites (kb from the 5' end): BamHI--3.02; BglII--5.39; EcoRI--0.91, 4.91; SacI--0.57, 4.19, 5.86; SalI--1.80, 3.91; SmaI--4.43.
Vector containing a non-autonomous P-element and incorporating the rough gene (ro) as a dominant marker for P-element mediated gene transfer into the germ line of Drosophila melanogaster.
The order of the major features in this plasmid is: HindIII - P-element/Carnegie 4/P-element - XhoI/MCS/SphI - ro gene.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Lockett TJ, et al. The rough (ro+) gene as a dominant P-element marker in germ line transformation of Drosophila melanogaster. Gene 114: 187-193, 1992. PubMed: 1601302

Trevor J Lockett, personal communication

Shipped freeze-dried