pJAK14 [JK388] (ATCC® 77289)

Applications: expression vectorshuttle vector  /  Depositors: JA Kornacki

Designations pJAK14 [JK388]
Permits and Restrictions

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Depositors JA Kornacki
Biosafety Level 1
Distribution host: Escherichia coli SCS1
Vector Information
Size (kb): 11.0000000000000000
Intact vector size: 11.000
Type of vector: plasmid
Cloning sites: EcoRI SacI Ecl136II KpnI BamHI XbaI HindIII
Polylinker sites: EcoRI SacI Ecl136II KpnI XmaI SmaI BamHI XbaI AccI SalI
HincII PstI SphI HindIII
Construction: pMMB67EH (ATCC 37622), Tn5
Host range: broad host range; Escherichia coli
Features (with orientation and position when available):
promoter: tac
MCS: EcoRI...HindIII, ->
transcription terminator: rrnB
marker(s): kanR (neoR)
replicon: oriV
replicon: oriT
replicon: RSF1010
repressor gene: lacIq
Vector: pJAK14 (plasmid)
Promoters: Promoter tac
Construction: pMMB67EH (ATCC 37622), Tn5
Marker(s):kanR (neoR)
Construct size (kb): 11.0
Features: marker(s): kanR (neoR)
promoter: tac
replicon: RSF1010
replicon: oriT
replicon: oriV
repressor gene: lacIq
MCS: EcoRI...HindIII
transcription terminator: rrnB
expression vector
shuttle vector
Restriction digests of the clone give the following sizes (kb): EcoRI--11.0; HindIII--11.0; PstI--8.2, 1.85, 1.0.
A shuttle vector permitting autoregulated high-level expression in all gram-negative bacteria.
Compatible with common E. coli cloning vectors, as well as vectors derived from IncP and IncW plasmids.
Can be mobilized by conjugative IncP helper plasmids [e.g. pRK2013 (ATCC 37159), pUB307] into Klebsiella aerogenes, Proteus mirabilis, Pseudomonas aeruginosa, Serratia marcescens, and other gram-negative bacteria.
Belongs to a group of vectors (ATCC 37622-37623, 77287-77292) differing only in antibiotic resistance and polylinker orientation.
Differs from pJAK15 (ATCC 77290) only in the orientation of the polylinker.
Media ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 37.0°C

Furste JP, et al. Molecular cloning of the plasmid RP4 primase region in a multi-host-range tacP expression vector. Gene 48: 119-131, 1986. PubMed: 3549457

Jon A Kornacki, personal communication

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