pUN70 [Y302, pNN336] (ATCC® 77268)

Applications: YC-type (centromeric) shuttle vectormutation detectionshuttle vectorvector permitting production of single-stranded DNAvector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase  /  Depositors: SJ Elledge

Designations pUN70 [Y302, pNN336]
Depositors SJ Elledge
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 5.6999998092651370
Vector: pUN70 (phagemid)
Promoters: Promoter lac
Construction: pUC18
Construct size (kb): 5.699999809265137
Features: insert detection: lacZ'
marker(s): URA3
marker(s): ampR
promoter: lac
replicon: ARS1
replicon: M13+
replicon: pMB1
MCS: EcoRI...HindIII
centromere: CEN4
YC-type (centromeric) shuttle vector
mutation detection
shuttle vector
vector permitting production of single-stranded DNA
vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase
Restriction digests of the clone give the following sizes (kb): EcoRI--5.2; KpnI--5.2; BamHI--5.2; PvuII--5.0, 0.3.
YC-type shuttle vector permitting visual detection of recombinants by lacZ alpha complementation and production of ssDNA (in the same sense as lacZ mRNA).
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Elledge SJ, Davis RW. A family of versatile centromeric vectors designed for use in the sectoring-shuffle mutagenesis assay in Saccharomyces cerevisiae. Gene 70: 303-312, 1988. PubMed: 3063604

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