pNC3 (ATCC® 77217)

Applications: contains sequence protease, neutralexpression vectorvector permitting visual detection of recombinantsvector with sequences for protein export  /  Depositors: L Wang

Designations pNC3
Depositors L Wang
Biosafety Level 1
Vector Information
Size (kb): 5.3540000915527340
Vector: pNC3 (plasmid)
Promoters: Promoter nprE
Construction: pWL267, pUC19
Construct size (kb): 5.349999904632568
Features: insert detection: protease
marker(s): kanR
promoter: nprE
replicon: pUB110
contains sequence protease, neutral
expression vector
vector permitting visual detection of recombinants
vector with sequences for protein export
Restriction digests of the clone give the following sizes (kb): BamHI/EcoRI--4.1, 1.15; HindIII--5.2; SalI--5.2; PstI--5.2; EcoRI--5.2.
Expression vector permitting visual detection of recombinants when plated on skim milk indicator plates by the loss or reduction of a halo around a colony.
Contains the signal sequence for the neutral protease, so that recombinants in-frame with the gene may be exported.
Constructed by an in-frame replacement of part of the pro-peptide coding region with part of the MCS from pUC19.
The order of the major features in this plasmid is: nprE (signal peptide coding region - MCS - pro peptide coding region - mature peptide coding region) - ori - kanR.
Media ATCC® Medium 1638: LB medium (ATCC medium 1065) with 5 mcg/ml kanamycin
Growth Conditions
Temperature: 37.0°C

Wu ZR, et al. Development of a novel Bacillus subtilis cloning system employing its neutral protease as screen marker. Gene 106: 103-107, 1991. PubMed: 1937030

Shipped freeze-dried