Size (kb): 4.6100001335144040
Vector: pFL38 (plasmid)
Promoters: Promoter lac
Construct size (kb): 4.610000133514404
Features: insert detection: lacZ'
marker(s): ampR, URA3
replicon: pMB1, ARS-CEN6
YC-type (centromeric) shuttle vector
vector permitting visual detection of recombinants
Restriction digests of the clone give the following sizes (kb): BamHI--4.8; BglII--3.7, 1.2; ClaI--4.0, 0.8.
The loss per generation (percentage having lost all the plasmids after 20 h of non-selective growth) is 7.3.
YC-type low copy number shuttle vector permitting visual detection of recombinants based on alpha complementation.
The order of the major features in this plasmid is: EcoRI/MCS/HindIII - URA3 - ARS-CEN6 - pMB1 ori - ampR.
Broach JR, et al. Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene. Gene 8: 121-133, 1979. PubMed: 395029
Bonneaud N, et al. A family of low and high copy replicative, integrative and single-stranded S. cerevisiae/E. coli shuttle vectors. Yeast 7: 609-615, 1991. PubMed: 1767589