pADbeta (ATCC® 77179)

Applications: contains easily purifiable cassette(s) for constructionreporter constructvector for other usesvector permitting visual detection of activity in colonies  /  Depositors: GR Macgregor

Permits and Restrictions

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Designations pADbeta
Depositors GR Macgregor
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 7.1999998092651370
Vector: pADbeta (plasmid)
Promoters: Promoter Ad2 major late
Construction: pUC19, pC4AUG beta-gal, p91023(B)
Marker(s):ampR
Construct size (kb): 7.199999809265137
Features: insert detection: lacZ'
marker(s): ampR
promoter: Ad2 major late
replicon: pMB1
terminator: SV40 late
Applications
contains easily purifiable cassette(s) for construction
reporter construct
vector for other uses
vector permitting visual detection of activity in colonies
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--7.2; HindIII--3.9, 3.3; EcoRI/HindIII--3.9, 2.7, 0.6; NotI--3.6 (doublet); BamHI--7.0, 0.2.
Reporter plasmid permitting visual detection of beta-galactosidase activity by histochemical staining.
Designed primarily as an enhancer trap vector. May be used as a reference plasmid in transfection experiments or to monitor the effect of trans-acting factors.
The lacZ gene may be excised by NotI and replaced with alternative sequences.
The order of the major features in this plasmid is: pUC19 - EcoRI - adenovirus 2 major late promoter/tripartite leader/splice signal - NotI - lacZ - NotI - SV40 late polyadenylation signal - XbaI - HindIII - pUC19.
Media ATCC® Medium 1945: Terrific broth (ATCC medium 1743) with 200 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

MacGregor GR, Caskey CT. Construction of plasmids that express E. coli beta-galactosidase in mammalian cells. Nucleic Acids Res. 17: 2365, 1989. PubMed: 2495524

Shipped freeze-dried
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