Size (kb): 7.1999998092651370
Vector: pADbeta (plasmid)
Promoters: Promoter Ad2 major late
Construction: pUC19, pC4AUG beta-gal, p91023(B)
Construct size (kb): 7.199999809265137
Features: insert detection: lacZ'
promoter: Ad2 major late
terminator: SV40 late
contains easily purifiable cassette(s) for construction
vector for other uses
vector permitting visual detection of activity in colonies
Restriction digests of the clone give the following sizes (kb): EcoRI--7.2; HindIII--3.9, 3.3; EcoRI/HindIII--3.9, 2.7, 0.6; NotI--3.6 (doublet); BamHI--7.0, 0.2.
Reporter plasmid permitting visual detection of beta-galactosidase activity by histochemical staining.
Designed primarily as an enhancer trap vector. May be used as a reference plasmid in transfection experiments or to monitor the effect of trans-acting factors.
The lacZ gene may be excised by NotI and replaced with alternative sequences.
The order of the major features in this plasmid is: pUC19 - EcoRI - adenovirus 2 major late promoter/tripartite leader/splice signal - NotI - lacZ - NotI - SV40 late polyadenylation signal - XbaI - HindIII - pUC19.
MacGregor GR, Caskey CT. Construction of plasmids that express E. coli beta-galactosidase in mammalian cells. Nucleic Acids Res. 17: 2365, 1989. PubMed: 2495524