pSVbeta plasmid in E. coli
Size (kb): 7.00
Vector: pSVbeta (plasmid)
Promoters: Promoter SV40 early
Construction: pUC19, pC4AUG beta-gal, pL1
Construct size (kb): 7.0
Features: insert detection: lacZ'
promoter: SV40 early
terminator: SV40 late
enhancer: SV40 early
contains easily purifiable cassette(s) for construction
vector for other uses
vector permitting visual detection of activity in colonies
Restriction digests of the clone give the following sizes (kb): EcoRI--7.0; HindIII--7.0; EcoRI/HindIII--4.3, 2.7; NotI--3.6 (doublet); BamHI--3.6, 3.2, 0.2.
Reporter plasmid permitting visual detection of beta-galactosidase activity by histochemical staining or fluorometric assay.
Designed to express high levels of beta-galactosidase or an alternative gene product in COS cells. May be used as a reference plasmid in transfection experiments or to monitor the effect of trans-acting factors.
The lacZ gene may be excised by NotI and replaced with alternative sequences.
Recommended ampicillin concentration is 200 ug/ml.
The order of the major features in this plasmid is: pUC19 - EcoRI - SV40 enhancer - SV40 early promoter - SV40 late 16S/19S splice signals - NotI - lacZ - NotI - SV40 late polyadenylation signal - HindIII - pUC19.
MacGregor GR, Caskey CT. Construction of plasmids that express E. coli beta-galactosidase in mammalian cells. Nucleic Acids Res. 17: 2365, 1989. PubMed: 2495524
Grant R Macgregor, personal communication