YRp14-trp1delta63 [YRp14/trp1-delta63, p430] (ATCC® 77148)

Applications: vector for other uses  /  Depositors: P Hieter

Permits and Restrictions

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Designations YRp14-trp1delta63 [YRp14/trp1-delta63, p430]
Depositors P Hieter
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Host
Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 8.3999996185302730
DESCRIPTION OF VECTOR:
Intact vector size: 8.400
Type of vector: plasmid
Cloning sites:
Polylinker sites:
Construction: YRp14
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, URA3, SUP11
replicon: pMB1, ARS1
Vector: YRp14-trp1delta63 (plasmid)
Construction: YRp14
Marker(s):URA3,ampR,SUP11
Construct size (kb): 8.399999618530273
Features: marker(s): ampR, URA3, SUP11
replicon: pMB1, ARS1
Applications
vector for other uses
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--6.0, 1.6, 0.6; HindIII--8.4; BamHI--8.4; PstI--3.7, 3.4, 1.3; BglII--7.4, 1.1.
A marker addition/transplacement vector.
Non-integrants grow poorly selecting for Ura3+ (pinpoints at 5 days) while integrants grow well because the SUP11 copy number is maintained at one. If there are no integrants on a transformation plate, streak onto -URA plates and pick papillae.
Constructed by inserting the equivalent of 2 genomic EcoRI/HindIII fragments (1.5 kb, centromere proximal and 0.8 kb, 3' end) from the TRP1 region into YRp14.
Represents the deletion of a 0.6 kb EcoRI/HindIII fragment containing the promoter and the 5' portion of the gene.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436

Philip Hieter, personal communication

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
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