YRp14-trp1delta63 [YRp14/trp1-delta63, p430] (ATCC® 77148)

Applications: vector for other uses  /  Depositors: P Hieter

Designations YRp14-trp1delta63 [YRp14/trp1-delta63, p430]
Permits and Restrictions

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Depositors P Hieter
Biosafety Level 1
Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 8.3999996185302730
Intact vector size: 8.400
Type of vector: plasmid
Cloning sites:
Polylinker sites:
Construction: YRp14
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, URA3, SUP11
replicon: pMB1, ARS1
Vector: YRp14-trp1delta63 (plasmid)
Construction: YRp14
Construct size (kb): 8.399999618530273
Features: marker(s): ampR, URA3, SUP11
replicon: pMB1, ARS1
vector for other uses
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--6.0, 1.6, 0.6; HindIII--8.4; BamHI--8.4; PstI--3.7, 3.4, 1.3; BglII--7.4, 1.1.
A marker addition/transplacement vector.
Non-integrants grow poorly selecting for Ura3+ (pinpoints at 5 days) while integrants grow well because the SUP11 copy number is maintained at one. If there are no integrants on a transformation plate, streak onto -URA plates and pick papillae.
Constructed by inserting the equivalent of 2 genomic EcoRI/HindIII fragments (1.5 kb, centromere proximal and 0.8 kb, 3' end) from the TRP1 region into YRp14.
Represents the deletion of a 0.6 kb EcoRI/HindIII fragment containing the promoter and the 5' portion of the gene.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436

Philip Hieter, personal communication

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