pUB110cop1 (ATCC® 77133)

Applications: shuttle vectorvector with low copy number  /  Depositors: H Leonhardt

Permits and Restrictions

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Designations pUB110cop1
Depositors H Leonhardt
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 4.5000000000000000
Vector: pUB110cop1 (plasmid)
Promoters: Promoter none
Construction: pUB110, pEB114cop1
Construct size (kb): 4.5
Features: marker(s): kanR, neoR
promoter: none
replicon: pUB110
shuttle vector
vector with low copy number
Restriction digests of the clone give the following sizes (kb): EcoRI--4.5; NcoI--4.5; BamHI--4.5.
The copy number is 11 +/- 2 in Bacillus subtilis, resulting in an increase in structural and segregational stability.
Low copy number shuttle vector.
Constructed by replacing the NcoI/AflII fragment of pUB110 (nt 4033-4543) with the corresponding fragment from pEB114cop1 which contains a G -> T transversion in the Shine-Dalgarno sequence of repU at position 4130.
Media ATCC Medium 1065 (see below) plus kanamycin/neomycin (3 mcg/ml) ATCC Medium 1065: Tryptone (Difco 0123), 10.0 g Yeast Extract (Difco 0127), 5.0 g NaCl, 10.0 g Distilled water, 1.0 L
Growth Conditions
Temperature: 37.0°C

Gryczan TJ, et al. Characterization of Staphylococcus aureus plasmids introduced by transformation into Bacillus subtilis. J. Bacteriol. 134: 318-329, 1978. PubMed: 418061

Gryczan T, et al. Characterization of chimeric plasmid cloning vehicles in Bacillus subtilis. J. Bacteriol. 141: 246-253, 1980. PubMed: 6243622

Leonhardt H. Identification of a low-copy-number mutation within the pUB110 replicon and its effect on plasmid stability in Bacillus subtilis. Gene 94: 121-124, 1990. PubMed: 2121616

Shipped freeze-dried