pASZ10 (ATCC® 77127)

Applications: YR-type (replicating) shuttle vectorcontains easily purifiable cassette(s) for constructioncontains sequence phosphoribosylaminoimidazole succinocarboxamide synthetase SAICAR synthetase, phosphoribosylaminoimidazole carboxylaseexpression vectorshuttle vectorvector containing primer sites useful for sequencingvector permitting RNA synthesis in vitrovector permitting production of single-stranded DNAvector permitting visual detection of activity in coloniesvector permitting visual detection of recombinants  /  Depositors: A Stotz

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Designations pASZ10
Depositors A Stotz
Biosafety Level 1
Vector Information
Size (kb): 5.3959999084472660
Vector: pASZ10 (phagemid)
Promoters: Promoter T7
Construction: pTZ19B, ADE2#0
Marker(s):ampR,ADE2
Construct size (kb): 5.395999908447266
Features: insert detection: lacZ'
marker(s): ampR, ADE2
promoter: lac, T7
replicon: pMB1, ARS, f1
Applications
YR-type (replicating) shuttle vector
contains easily purifiable cassette(s) for construction
contains sequence phosphoribosylaminoimidazole succinocarboxamide synthetase SAICAR synthetase, phosphoribosylaminoimidazole carboxylase
expression vector
shuttle vector
vector containing primer sites useful for sequencing
vector permitting RNA synthesis in vitro
vector permitting production of single-stranded DNA
vector permitting visual detection of activity in colonies
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): BglII--2.9, 2.6; EcoRI--5.6; HindIII--2.9, 1.3, 1.1, 0.41; BamHI--5.6.
The ADE2 segment contains HindIII sites approximately 1.17 kb and 2.36 kb from the 5' BglII site.
A YR-type shuttle expression vector containing promoters for in vitro RNA synthesis and priming sites useful for sequencing. Contains readily purifiable elements for construction experiments.
In E. coli, permits visual detection of recombinants and production of ssDNA. Encodes the lacZalpha (lacZ') peptide.
The naturally occurring BglII site within ADE2 was destroyed by replacing the A at nt 1243 of the reported sequence with a G. A BglII linker was added to the 5' end,
and the naturally occurring BglII site at the 3' end (nt 2512) was used to create a BglII fragment containing the complete coding sequence.
pTZ19B was derived from pTZ19R by adding a BglII linker at coordinate 416.
Constructed by inserting a 2.5 kb fragment (Ars+ Ade2+) into the BglII site of pTZ19B.
The order of the major features in this plasmid is: lacZ'/MCS - T7 promoter - ADE2 - pMB1 ori - ampR - f1 ori.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Stotz A, Linder P. The ADE2 gene from Saccharomyces cerevisiae: sequence and new vectors. Gene 95: 91-98, 1990. PubMed: 2253890

Shipped freeze-dried
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