pBP109 (ATCC® 77089)

Applications: acentric fragmentation vectorshuttle vectorvector permitting RNA synthesis in vitro  /  Depositors: RH Reeves

Designations pBP109
Depositors RH Reeves
Biosafety Level 1
Vector Information
Size (kb): 4.9000000953674320
Vector: pBP109 (plasmid)
Promoters: Promoter lac
Construction: YTCA-1x, pRS303, BLUR8
Construct size (kb): 4.900000095367432
Features: marker(s): ampR, HIS3
promoter: lac, SP6
replicon: pMB1
acentric fragmentation vector
shuttle vector
vector permitting RNA synthesis in vitro
Restriction digests of the clone give the following sizes (kb): BamHI--4.6, 0.3; EcoRI--4.9; SmaI--4.9; PstI--3.4, 1.15, 0.35.
Acentric chromosome fragmentation vector targeting Alu sequences. Contains promoters for in vitro RNA synthesis.
Any unique restriction site between the targeting sequence and the telomere-adjacent sequence can be used to linearize the plasmid before transformation.
The EcoRV and ClaI sites between the SP6 promoter and the telomere sequence can be used for recovery from deletion derivatives of YAC insert sequences adjacent to the fragmentation site.
Constructed from pBP103 (ATCC 77087) by inserting a 0.3 kb Alu-containing sequence from BLUR8 into the BamHI site. pBP108 (ATCC 77088) and pBP109 (ATCC 77089) differ in the orientation of the Alu sequence.
The order of the major features in this plasmid is: bla - HIS3 - lacZ'/MCS/5'Alu3' - HindIII - SphI - PstI - AccI - SalI - TEL - ClaI - EcoRV - SP6 promoter.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Pavan WJ, et al. High-efficiency yeast artificial chromosome fragmentation vectors. Gene 106: 125-127, 1991. PubMed: 1937033

Reeves RH, et al. Yeast artificial chromosome modification and manipulation. Methods Enzymol. 216: 584-603, 1992. PubMed: 1336105

Shipped freeze-dried