pHCMV-G (ATCC® 75497)

Applications: cloning vector  /  Depositors: Regents of the University of California, J Yee, JC Burns, Regents of the University of California

Designations pHCMV-G
Permits and Restrictions

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Depositors Regents of the University of California, J Yee, JC Burns, Regents of the University of California
U.S. Patent
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Biosafety Level 1
Vector Information
Size (kb): 6.3499999046325680
DESCRIPTION OF VECTOR:
Intact vector size: 6.350
Type of vector: plasmid
Cloning sites: XbaI, SalI, BamHI, XhoI, KpnI
Polylinker sites:
Construction: pBR322, HCMV, VSV-G
Host range: Homo sapiens; hamster; zebrafish; Escherichia coli
Features (with orientation and position when available):
promoter: HCMV (cytomegalovirus), ->
marker(s): ampR, ->
Vector: pHCMV-G (plasmid)
Promoters: Promoter HCMV (cytomegalovirus)
Construction: pBR322, HCMV, VSV-G
Marker(s):ampR
Construct size (kb): 6.349999904632568
Features: marker(s): ampR
promoter: HCMV (cytomegalovirus)
Applications
cloning vector
Comments
The retrovirus-derived vectors in which the retroviral envelope glycoprotein has been replaced by the G glycoprotein of vesicular stomatitis virus. Vector can be used in the transfer of an exogenous gene into the cells of non-mammalian organisms.
Contains the immediate early promoter of human cytomegalovirus, the cDNA encoding VSC-G protein and the polyademylation signal of the rabbit B-globin gene.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Burns JC. Generation, concentration and efficient transfer of VSV-G pseudotyped retroviral vectors. US Patent 5,512,421 dated Apr 30 1996

Shipped frozen
Shipping Information Distributed: DNA supplied by depositor (500 ng dried)