4-1BBL (ATCC® 68682)

Depositors: Immunex Corporation, JB Oster, Immunex Corporation

Designations 4-1BBL
Permits and Restrictions

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Depositors Immunex Corporation, JB Oster, Immunex Corporation
U.S. Patent
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Biosafety Level 1
Vector Information
Construct size (kb): 7.199999809265137
Comments
The plasmid pDC201 was assembled from (1)the SV40 origin of replication, enhancerand early and late promoters; (2)the adenovirus-2 major late promoter and tripartite leader; (3)adeenovirus-2-virus-associated RNA genes (VAI/VAII) and, (4)pMSLV.
(5)SV40 polyadenylation and transcription termination signals. Restriction digests of the clone give the following sizes (kb): BamHI--3.8,2.5,0.63,0.3; PstI--7.0; XhoI--6.0,0.56.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Goodwin RA, et al. Cytokine designated 4-1BB ligand. US Patent 5,674,704 dated Oct 7 1997

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Shipping Information Distributed: freeze-dried