pSELECT-1 [pALTER-1] (ATCC® 68196)

Applications: vector containing primer sites useful for sequencingvector permitting RNA synthesis in vitrovector permitting visual detection of recombinantsvector useful for in-vitro mutagenesis  /  Depositors: Promega Corporation, JC Grosch, Promega Corporation

Designations pSELECT-1 [pALTER-1]
Permits and Restrictions

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Depositors Promega Corporation, JC Grosch, Promega Corporation
U.S. Patent
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Biosafety Level 1
Vector Information
Size (kb): 5.6799998283386230
Vector: pSELECT-1 (phagemid)
Promoters: Promoter T7
Construction: pBR322, pGEM-3Zf(+)
Construct size (kb): 5.679999828338623
Features: insert detection: lacZ'
marker(s): tetR
promoter: lac, SP6, T7
replicon: pMB1, f1
vector containing primer sites useful for sequencing
vector permitting RNA synthesis in vitro
vector permitting visual detection of recombinants
vector useful for in-vitro mutagenesis
Restriction digests of the clone give the following sizes (kb): EcoRI--6.2; BamHI--6.2; HindIII--6.2.
This vector contains the following unique restriction sites: ClaI, EcoRV, StyI, ScaI and AatII.
Useful for in vitro mutagenesis experiments. Encodes the beta-galactosidase alpha peptide, permitting blue-white visual detection. Can be used to produce ssDNA. Contains promoters for in vitro RNA synthesis and priming sites useful for sequencing.
Contains an inactive ampicillin resistance gene which can be reverted to functional expression by a repair oligonucleotide.
Media ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Growth Conditions
Temperature: 37.0°C

Promega Corporation, personal communication

Shipped frozen
Shipping Information

Shipped:  Freeze dried E. coli containing the plasmid