pKL19-2 (ATCC® 67997)

Applications: expression vectorvector permitting construction of fusion proteinsvector permitting visual detection of recombinants  /  Depositors: New England Biolabs, Inc., KD Lunnen, New England Biolabs, Inc.

Designations pKL19-2
Permits and Restrictions

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Depositors New England Biolabs, Inc., KD Lunnen, New England Biolabs, Inc.
U.S. Patent
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Biosafety Level 1
Vector Information
Size (kb): 2.6900000572204590
Vector: pKL19-2 (plasmid)
Promoters: Promoter lac
Construction: pUC19
Marker(s):ampR
Construct size (kb): 2.690000057220459
Features: insert detection: lacZ'
marker(s): ampR
promoter: lac
replicon: pMB1
terminator: none
enhancer: none
Applications
expression vector
vector permitting construction of fusion proteins
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): PstI--2.7; BamHI--2.7; HindIII--2.7; EcoRI--2.7.
Expression vector permitting production of a fusion protein and visual detection of recombinants,
using the beta-galactosidase alpha peptide as the reporter group.
Distributed in aliquots of 2 ug (100 ng/ul).
A pUC19 derivative with 2 XmaI sites--one at nt 412 in the multiple cloning site and one added at nt 1567 by replacing DNA between the DraI sites at nt 1563 and 1582 with phosphorylated d[pCCCCGGGG].
References

Lunnen KD, Wilson GG. Method for producing the XmaI restriction endonuclease and methylase. US Patent 5,002,882 dated Mar 26 1991

Keith D Lunnen, personal communication

Shipped freeze-dried