pKL19-2 (ATCC® 67997)

Applications: expression vectorvector permitting construction of fusion proteinsvector permitting visual detection of recombinants  /  Depositors: New England Biolabs, Inc., KD Lunnen, New England Biolabs, Inc.

Permits and Restrictions

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Designations pKL19-2
Depositors New England Biolabs, Inc., KD Lunnen, New England Biolabs, Inc.
Biosafety Level 1
Vector Information
Size (kb): 2.6900000572204590
Vector: pKL19-2 (plasmid)
Promoters: Promoter lac
Construction: pUC19
Marker(s):ampR
Construct size (kb): 2.690000057220459
Features: insert detection: lacZ'
marker(s): ampR
promoter: lac
replicon: pMB1
terminator: none
enhancer: none
Applications
expression vector
vector permitting construction of fusion proteins
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): PstI--2.7; BamHI--2.7; HindIII--2.7; EcoRI--2.7.
Expression vector permitting production of a fusion protein and visual detection of recombinants,
using the beta-galactosidase alpha peptide as the reporter group.
Distributed in aliquots of 2 ug (100 ng/ul).
A pUC19 derivative with 2 XmaI sites--one at nt 412 in the multiple cloning site and one added at nt 1567 by replacing DNA between the DraI sites at nt 1563 and 1582 with phosphorylated d[pCCCCGGGG].
References

Lunnen KD, Wilson GG. Method for producing the XmaI restriction endonuclease and methylase. US Patent 5,002,882 dated Mar 26 1991

Keith D Lunnen, personal communication

Shipped frozen
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