#595 [S-595] (ATCC® 67755)

Applications: contains sequence interleukin 1, beta  /  Depositors: Otsuka Pharmaceuticals Co., Ltd., PM Arthur, Otsuka Pharmaceuticals Co., Ltd.

Designations #595 [S-595]
Permits and Restrictions

View Permits

Depositors Otsuka Pharmaceuticals Co., Ltd., PM Arthur, Otsuka Pharmaceuticals Co., Ltd.
U.S. Patent
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Biosafety Level 1
Vector Information
Size (kb): 4.5560002326965330
Vector: #595 (plasmid)
Promoters: Promoter for expression lambda PR
Construct size (kb): 4.556000232696533
Features: marker(s): ampR
promoter for expression: lambda PR
replicon: pMB1
repressor gene: cI857
restriction site: BamHI
restriction site: ClaI
terminator: lambda 4s
coding sequence: IL-1, mature peptide
contains sequence interleukin 1, beta
A putative RBS can be cloned into the ClaI site using a double stranded oligonucleotide having a GC overhang at the 3' end.
The vector allows testing of various oligonucleotide sequences as suitable ribosome binding sites (RBS) for expression of the gene in prokaryotic cells. The plasmid construct lacks a 5' leader sequence and RBS.
The optimal RBS may vary from protein to protein. To facilitate testing other proteins, the insert coding sequence can be replaced by other foreign gene sequences, using ClaI and BamHI. The foreign gene must provide its own ATG initiation codon.
Recommendation for verification: BamHI+ClaI, BglI, PstI.
Media ATCC® Medium 1315: LB Medium (ATCC medium 1065) with 100 mg/L ampicillin
Growth Conditions
Temperature: 30.0°C

Arthur PM, Duckworth BC. Gene regulation cassettes, expression vectors containing the same and microorganisms transformed with the same. US Patent 5,427,924 dated Jun 27 1995