pLS21 [1002] (ATCC® 67492)

Applications: expression vectorshuttle vectorvector containing primer sites useful for sequencingvector permitting RNA synthesis in vitro  /  Depositors: Brookhaven National Laboratory, SA Lacks, Brookhaven National Laboratory

Permits and Restrictions

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Designations pLS21 [1002]
Depositors Brookhaven National Laboratory, SA Lacks, Brookhaven National Laboratory
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 8.1000003814697270
Vector: pLS21 (plasmid)
Promoters: Promoter T7 (phi10)
Construction: pAR2192
Marker(s):ampR,tetR
Construct size (kb): 8.100000381469727
Features: marker(s): ampR, tetR
promoter: T7 (phi10)
replicon: pMB1, rep
Applications
expression vector
shuttle vector
vector containing primer sites useful for sequencing
vector permitting RNA synthesis in vitro
Comments
Strain is noncapsulated and never reverts to encapsulated form.
Should be treated as a class II biohazard.
The HindIII site is downstream of the T7 promoter.
Media ATCC® Medium 1420: Trypticase soy agar with defibrinated sheep blood, sucrose and 0.5 mcg/ml tetracycline
Growth Conditions
Temperature: 37.0°C
References

Lacks SA. Recombinant plasmids for encoding restriction enzymes DpnI and DpnII of Streptococcus pneumoniae. US Patent 4,960,707 dated Oct 2 1990

Lacks SA, et al. Identification and analysis of genes for tetracycline resistance and replication functions in the broad-host-range plasmid pLS1. J. Mol. Biol. 192: 753-765, 1986. PubMed: 2438417

Sanford A Lacks, personal communication

Shipped freeze-dried
Shipping Information

Shipped:  Freeze-dried Streptococcus pneumoniae containing the plasmid.

Product Sheet
Product Sheet
Product Sheet