pYAC2 (ATCC® 67380)

Applications: YL-type (YAC) shuttle vectorshuttle vectorvector for constructing genomic libraries  /  Depositors: MV Olson

Permits and Restrictions

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Designations pYAC2
U.S. Patent
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished.
Depositors MV Olson
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 11.1000003814697300
Vector: pYAC2 (YAC)
Marker(s):TRP1,URA3,ampR,HIS3,SUP4
Construct size (kb): 11.10000038146973
Features: marker(s): ampR, HIS3, SUP4, TRP1, URA3
replicon: pMB1, ARS1
centromere: CEN4
Applications
YL-type (YAC) shuttle vector
shuttle vector
vector for constructing genomic libraries
Comments
A yeast artificial chromosome vector for cloning blunt-ended fragments.
This yeast artificial chromosome contains ARS1, CEN4 and Tetrahymena telomeric elements.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Burke DT, et al. Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors. Science 236: 806-812, 1987. PubMed: 3033825

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet