pBC102-K22 (ATCC® 67255)

Applications: YE-type (episomal) shuttle vectorexpression vectorvector permitting construction of fusion proteinsvector with sequences for protein export  /  Depositors: Immunex Corporation, SG Hallquist, Immunex Corporation

Permits and Restrictions

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Designations pBC102-K22
U.S. Patent
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished.
Depositors Immunex Corporation, SG Hallquist, Immunex Corporation
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 9.3999996185302730
Vector: pBC102-K22 (plasmid)
Promoters: Promoter ADH2
Construction: pBR322
Construct size (kb): 9.399999618530273
Features: marker(s): ampR, TRP1
promoter: ADH2
replicon: pMB1, 2 micron
YE-type (episomal) shuttle vector
expression vector
vector permitting construction of fusion proteins
vector with sequences for protein export
Restriction digests of the clone give the following sizes (kb): EcoRI--9.4; BamHI--uncut; HindIII--6.5, 2.1, 0.8; PstI--5.8, 1.9, 1.7.
Shuttle expression vector utilizing sequences encoding the alpha factor leader peptide to allow secretion of recombinant proteins.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Curtis BM, et al. Fusion proteins comprising GM-CSF and IL-3. US Patent 5,073,627 dated Dec 17 1991

Curtis BM, et al. DNA sequences encoding fusion proteins comprising GM-CSF and IL-3. US Patent 5,108,910 dated Apr 28 1992

Shipped frozen
Shipping Information Distributed: freeze-dried