pHU10 plasmid in E. coli (ATCC® 57002)

Depositors: TP Dryja

Designations pHU10 plasmid in E. coli
Permits and Restrictions

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Depositors TP Dryja
Biosafety Level 1
Host Escherichia coli K12
Vector Information Total size of construct (kb): 7.2
Marker: ampR
Enzyme to excise insert: HindIII

Vector: pBR322
Vector type: plasmid
Vector size (kb): 4.363
Markers: ampR
Vector ends: HindIII
Restriction digests of the clone give the following sizes (kb): HindIII--4.4, 2.8; PstI--7.2.
A multiple site polymorphism also detected with XmnI and EcoRI, with a common band of 6.0 and alleles of 8.5 and 10 kb.
An unpublished RFLP is detected by this probe when genomic DNA is digested with EcoRI. The published RFLP is revealed with XmnI.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37°C

Dryja TP, et al. Chromosome 13 restriction fragment length polymorphisms. Hum. Genet. 65: 320-324, 1984. PubMed: 6319270

Dryja TP, Morton CC. Mapping of seven polymorphic loci on human chromosome 13 by in situ hybridization. Hum. Genet. 71: 192-195, 1985. PubMed: 4065891

Misra BC, Srivatsan ES. Localization of HeLa cell tumor-suppressor gene to the long arm of chromosome II. Am. J. Hum. Genet. 45: 565-577, 1989. PubMed: 2577469

Lundberg C, et al. Loss of heterozygosity in human ductal breast tumors indicates a recessive mutation on chromosome 13. Proc. Natl. Acad. Sci. USA 84: 2372-2376, 1987. PubMed: 3031679

Leppert M, et al. A primary genetic map of chromosome 13q. Am. J. Hum. Genet. 39: 425-437, 1986. PubMed: 3464200

Thaddeus P Dryja, personal communication

Shipped freeze-dried
Shipping Information Freeze-dried E. coli containing the plasmid