pGL2B (ATCC® 53366)

Applications: expression vectorvector with sequences for protein export  /  Depositors: Gist-Brocades N.V., DI Gwynne, Gist-Brocades N.V.

Designations pGL2B
Permits and Restrictions

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Depositors Gist-Brocades N.V., DI Gwynne, Gist-Brocades N.V.
U.S. Patent
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Biosafety Level 1
Distribution host: Escherichia coli JM83; K-12 strain JM83
Vector Information
Size (kb): 6.6
Vector: pGL2B (plasmid)
Promoters: Promoter glucoamylase
Construction: pUC12
Construct size (kb): 6.6
Features: marker(s): ampR
promoter: glucoamylase
replicon: pMB1
signal peptide coding region: glucoamylase
expression vector
vector with sequences for protein export
Restriction digests of the clone give the following sizes (kb): BamHI--6.8; BglII--6.8; EcoRI--4.5, 1.8, 0.32.
One of 3 vectors (pGL2A ATCC 53365, pGL2B 53366, pGL2C ATCC 53367) differing in the length of a synthetic linker and thus providing alternate reading frames.
Constructed by inserting a HindIII/BglII fragment containing the glucoamylase gene into pUC12. A BssHII/SstI fragment (starting just 3' to the region coding the signal peptide) was then replaced with a synthetic linker having EcoRV and BglII sites.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Gwynne DI, et al. Vectors in use in filamentous fungi. US Patent 5,198,345 dated Mar 30 1993

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