pGL2B (ATCC® 53366)

Applications: expression vectorvector with sequences for protein export  /  Depositors: Gist-Brocades N.V., DI Gwynne, Gist-Brocades N.V.

Designations pGL2B
Depositors Gist-Brocades N.V., DI Gwynne, Gist-Brocades N.V.
U.S. Patent
Disclosure FPAT
Biosafety Level 1
Distribution host: Escherichia coli JM83; K-12 strain JM83
U.S. Patent
Vector Information
Size (kb): 6.6
Vector: pGL2B (plasmid)
Promoters: Promoter glucoamylase
Construction: pUC12
Construct size (kb): 6.6
Features: marker(s): ampR
promoter: glucoamylase
replicon: pMB1
signal peptide coding region: glucoamylase
expression vector
vector with sequences for protein export
Restriction digests of the clone give the following sizes (kb): BamHI--6.8; BglII--6.8; EcoRI--4.5, 1.8, 0.32.
One of 3 vectors (pGL2A ATCC 53365, pGL2B 53366, pGL2C ATCC 53367) differing in the length of a synthetic linker and thus providing alternate reading frames.
Constructed by inserting a HindIII/BglII fragment containing the glucoamylase gene into pUC12. A BssHII/SstI fragment (starting just 3' to the region coding the signal peptide) was then replaced with a synthetic linker having EcoRV and BglII sites.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Gwynne DI, et al. Vectors in use in filamentous fungi. US Patent 5,198,345 dated Mar 30 1993

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