palpha3 (ATCC® 53220)

Applications: YE-type (episomal) shuttle vectorexpression vectorshuttle vector  /  Depositors: Immunex Corporation, S Gillis, Immunex Corporation

Permits and Restrictions

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Designations palpha3
Depositors Immunex Corporation, S Gillis, Immunex Corporation
Biosafety Level 1
Host
Distribution host: Escherichia coli RR1; K-12 RR1
Vector Information
Size (kb): 7.5999999046325680
Vector: palpha3 (plasmid)
Promoters: Promoter alpha factor
Construction: pBR322, pYalphaGM-2
Marker(s):TRP1,ampR
Construct size (kb): 7.599999904632568
Features: marker(s): ampR, TRP1
promoter: alpha factor
replicon: pMB1, 2 micron
Applications
YE-type (episomal) shuttle vector
expression vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): KpnI--7.6; PstI--3.3, 1.8, 1.6, 0.8; EcoRI--7.6; HindIII--5.4, 2.0; BamHI--uncut.
The EcoRI/KpnI promoter cassette contains the pre-pro alpha factor promoter and leader plus ATG.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Anderson DM, et al. DNA sequences encoding bovine interleukin-2. US Patent 4,882,282 dated Nov 21 1989

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
Product Sheet
Product Sheet
Product Sheet