pHV33 (ATCC® 39217)

Applications: shuttle vector  /  Depositors: Miles Laboratories, Inc., KL Wollweber, Miles Laboratories, Inc.

Designations pHV33
Permits and Restrictions

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Depositors Miles Laboratories, Inc., KL Wollweber, Miles Laboratories, Inc.
U.S. Patent
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Biosafety Level 1
Host
Distribution host: Escherichia coli RR1; K-12 RR1
Vector Information
Size (kb): 6.9000000953674320
Vector: pHV33 (plasmid)
Marker(s):ampR,cmlR,tetR
Construct size (kb): 6.900000095367432
Features: marker(s): ampR, cmlR, tetR
replicon: pMB1
Applications
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--7.9; HindIII--4.65, 3.05.
This is an unstable, tetracycline-resistant mutant of pHV14.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Primrose SB, Ehrlich SD. Isolation of plamid deletion mutants and study of their instability. Plasmid 6: 193-201, 1981. PubMed: 7029580

Shipped freeze-dried
Shipping Information Distributed: freeze-dried