pCB101 (ATCC® 39020)

Applications: shuttle vector  /  Depositors: Kyowa Ferm. Ind. Co., Ltd., S Wakaki, Kyowa Ferm. Ind. Co., Ltd.

Designations pCB101
Permits and Restrictions

View Permits

Depositors Kyowa Ferm. Ind. Co., Ltd., S Wakaki, Kyowa Ferm. Ind. Co., Ltd.
U.S. Patent
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Biosafety Level 1
Host
Distribution host: Corynebacterium glutamicum LA103
Vector Information
Size (kb): 11.3000001907348600
Vector: pCB101 (plasmid)
Construction: pCG11 (ATCC 39022), pUB110 (ATCC 37015)
Marker(s):kanR,spcR
Construct size (kb): 11.30000019073486
Features: marker(s): kanR, spcR
replicon: pCG1, pUB110
Applications
shuttle vector
Comments
The original vials from the depositor are being used for distribution.
Cloning into the BglII site inactivates kanR.
This was constructed by ligating pCG11 (ATCC 39022) linearized with BglII to pUB110 linearized with BamHI.
Media ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 30.0°C
References

Katsumata R, et al. Novel vector plasmids. US Patent 4,710,471 dated Dec 1 1987

Shipped frozen
Shipping Information Distributed: freeze-dried