pHS24 (ATCC® 37841)

Applications: contains sequence alcohol dehydrogenaseintegrating vectorshuttle vector  /  Depositors: HG Sass

Permits and Restrictions

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Designations pHS24
Depositors HG Sass
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 12.0000000000000000
Vector: pHS24 (plasmid)
Promoters: Promoter hsp82
Construction: pHS17
Marker(s):G418R,ampR,Adh+
Construct size (kb): 12.0
Features: marker(s): ampR, G418R, Adh+
promoter: hsp82
replicon: pMB1
terminator: hsp82
Applications
contains sequence alcohol dehydrogenase
integrating vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): KpnI--12.5; XbaI--11.0, 0.72, 0.50, 0.37; BamHI/KpnI--7.6, 3.8, 1.3.
Expression is constitutive, but can be increased with heat.
Selection of transgenic Drosophila by G418 has been done with embryos, larvae, and adults. Adults can be reselected by 6% ethanol.
Can be used in Drosophila spp. cell culture transfection assays, and may function in other animal and plant systems because the hsp82 promoter is highly conserved.
P-transposable (integrating) shuttle vector.
Contains hsp82-neo fusion gene which encodes the first 20 aa of D. pseudoobscura hsp82, 5 codons in a linker, fused to the fifth codon of bacterial neomycin phosphotransferase from Tn5, and followed by polyadenylation signals from D. melanogaster hsp82.
Also contains a complete alcohol dehydrogenase coding region from D. melanogaster (3.2 kb XbaI fragment) which includes 0.66 kb of 5' and 0.64 kb of 3' DNA, and has all the cis-acting sequences necessary for expression.
The order of the major components of this construct are: 3' P-element, 5'-Adh-3', 5'-hsp82-neo fusion-hsp82 polyadenylation signal-3', KpnI site, and 5' P-element.
Differs from pHS22 (ATCC 37840) by the removal of hsp82 upstream sequences from approximately -5000 to -868 (where the transcription start point is +1).
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Sass H. P-transposable vectors expressing a constitutive and thermoinducible hsp82-neo fusion gene for Drosophila germline transformation and tissue-culture transfection. Gene 89: 179-186, 1990. PubMed: 2165019

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