Size (kb): 7.0999999046325680
Vector: YIp356R (plasmid)
Promoters: Promoter none
Construction: YEp356R (ATCC 37737)
Construct size (kb): 7.099999904632568
Features: insert detection: lacZ
marker(s): ampR, URA3
YI-type (integrating) shuttle vector
The cleavage position in the reading frame for cloning sites is (where 3 = between triplets): HindIII-1; SphI-2; PstI-2; SalI-1; XbaI-1; BamHI-1; SmaI-2; KpnI-2; SacI-2; EcoRI-1. The SacI site is not unique.
Restriction digests of the clone give the following sizes (kb): EcoRI--7.1; SalI--7.1; PstI--7.1; HindIII--7.1.
One of 3 promoter-cloning, YI type shuttle vectors (ATCC 37755
- 37757) with URA3 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter gene and multiple cloning sites differing in reading frame.
The sequence and reading frame of the multiple cloning sequence is: 5'AAG CTT GCA TGC CTG CAG GTC GAC TCT AGA GGA TCC CCG GGT ACC GAG CTC GAA TTC CCA GCT TGC GAT CCC3', from nucleotide 1 of the MCS through CCC for amino acid 8 of beta-galactosidase.
Myers AM, et al. Yeast shuttle and integrative vectors with multiple cloning sites suitable for construction of lacZ fusions. Gene 45: 299-310, 1986. PubMed: 3026915