Size (kb): 7.0999999046325680
Vector: YIp358 (plasmid)
Promoters: Promoter none
Construction: YEp358 (ATCC 37733)
Construct size (kb): 7.099999904632568
Features: insert detection: lacZ
marker(s): ampR, URA3
YI-type (integrating) shuttle vector
The cleavage position in the reading frame for cloning sites is (where 3 = between triplets): EcoRI-3; SacI-1; KpnI-1; SmaI-3; BamHI-3; XbaI-3; SalI-3; PstI-1; SphI-1; HindIII-3. The SacI site is not unique.
Restriction digests of the clone give the following sizes (kb): EcoRI-7.1; SalI-7.1; PstI-7.1; HindIII-7.1.
One of 3 promoter-cloning, YI type shuttle vectors (ATCC 37749
- 37751) with URA3 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter gene and multiple cloning sites differing in reading frame.
The sequence and reading frame of the multiple cloning sequence is: 5'G AAT TCG AGC TCG GTA CCC GGG GAT CCT CTA GAG TCG ACC TGC AGG CAT GCA AGC TTC GAT CCC 3', from nucleotide 1 of the MCS through CCC for amino acid 8 of beta-galactosidase.
Myers AM, et al. Yeast shuttle and integrative vectors with multiple cloning sites suitable for construction of lacZ fusions. Gene 45: 299-310, 1986. PubMed: 3026915