Size (kb): 8.0000000000000000
Vector: YEp358R (plasmid)
Promoters: Promoter none
Construction: YEp358 (ATCC 37733)
Construct size (kb): 8.0
Features: insert detection: lacZ
marker(s): ampR, URA3
replicon: pMB1, 2 micron
YE-type (episomal) shuttle vector
Cloning into the HindIII, SphI, PstI, SalI, or XbaI sites leads to a TAG stop codon within the downstream XbaI site of the multiple cloning region.
The cleavage position in the reading frame for cloning sites is (where 3 = between triplets): HindIII-2; SphI-3; PstI-3; SalI-2; XbaI-2; BamHI-2; SmaI-3; KpnI-3; SacI-3; EcoRI-2. The SacI site is not unique.
Restriction digests of the clone give the following sizes (kb): HindIII--8.0; EcoRI--8.0; PstI--8.0.
One of 3 promoter-cloning, YE type shuttle vectors (ATCC 37737
- 37739) with URA3 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter gene and multiple cloning sites differing in reading frame.
The sequence and reading frame of the multiple cloning sequence is: 5'AA GCT TGC ATG CCT GCA GGT CGA CTC TAG AGG ATC CCC GGG TAC CGA GCT CGA ATT CCC AGC TTC GAT CCC3', from nucleotide 1 of the MCS through CCC for amino acid 8 of beta-galactosidase.
in E. coli containing the plasmid