YEp368 (ATCC® 37736)

Applications: YE-type (episomal) shuttle vectorpromoter-cloning vectorshuttle vector  /  Depositors: CJ Lusty

Permits and Restrictions

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Designations YEp368
Depositors CJ Lusty
Biosafety Level 1
Vector Information
Size (kb): 8.3999996185302730
Vector: YEp368 (plasmid)
Promoters: Promoter none
Construction: YEp363A, YEp358
Marker(s):LEU2,ampR
Construct size (kb): 8.399999618530273
Features: insert detection: lacZ
marker(s): ampR, LEU2
promoter: none
replicon: pMB1, 2 micron
Applications
YE-type (episomal) shuttle vector
promoter-cloning vector
shuttle vector
Comments
The cleavage position in the reading frame for cloning sites is (where 3 = between triplets): EcoRI-3; SacI-1; KpnI-1; SmaI-3; BamHI-3; XbaI-3; SalI-3; PstI-1; SphI-1; HindIII-3. The EcoRI, SacI, and Kpn I sites are not unique.
Restriction digests of the clone give the following sizes (kb): HindIII--8.4; BamHI--8.4; SalI--8.4; PstI--8.4; EcoRI--4.25, 4.15.
One of 3 promoter-cloning, YE type shuttle vectors (ATCC 37734 - 37736) with LEU2 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter gene and multiple cloning sites differing in reading frame.
The sequence and reading frame of the multiple cloning sequence is: 5'G AAT TCG AGC TCG GTA CCC GGG GAT CCT CTA GAG TCG ACC TGC AGG CAT GCA AGC TTC GAT CCC 3', from nucleotide 1 of the MCS through CCC for amino acid 8 of beta-galactosidase.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Myers AM, et al. Yeast shuttle and integrative vectors with multiple cloning sites suitable for construction of lacZ fusions. Gene 45: 299-310, 1986. PubMed: 3026915

Shipped freeze-dried
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