Size (kb): 8.3999996185302730
Vector: YEp366 (plasmid)
Promoters: Promoter none
Construction: YEp363A, YEp356
Construct size (kb): 8.399999618530273
Features: insert detection: lacZ
marker(s): ampR, LEU2
replicon: pMB1, 2 micron
YE-type (episomal) shuttle vector
The cleavage position in the reading frame for cloning sites is (where 3 = between triplets): EcoRI-2; SacI-3; KpnI-3; SmaI-2; BamHI-2; XbaI-2; SalI-2; PstI-3; SphI-3; HindIII-2. The EcoRI, SacI, and KpnI sites are not unique.
Cloning into the EcoRI, SacI, KpnI SmaI, BamHI, or XbaI sites leads to a TAG stop codon within the downstream XbaI site of the multiple cloning region.
Restriction digests of the clone give the following sizes (kb): HindIII--8.4; BamHI--8.4; SalI--8.4; PstI--8.4; EcoRI--4.25, 4.15.
One of 3 promoter-cloning, YE type shuttle vectors (ATCC 37734
- 37736) with LEU2 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter gene and multiple cloning sites differing in reading frame.
The sequence and reading frame of the multiple cloning sequence is: 5'GA ATT CGA GCT CGG TAC CCG GGG ATC CTC TAG AGT CGA CCT GCA GGC ATG CAA GCT TGC GAT CCC 3', from nucleotide 1 of the MCS through CCC for amino acid 8 of beta-galactosidase.
Myers AM, et al. Yeast shuttle and integrative vectors with multiple cloning sites suitable for construction of lacZ fusions. Gene 45: 299-310, 1986. PubMed: 3026915