pJ6omega (ATCC® 37723)

Applications: expression vectorshuttle vector  /  Depositors: H Land

Designations pJ6omega
Permits and Restrictions

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Depositors H Land
Biosafety Level 1
Vector Information
Size (kb): 3.5000000000000000
Vector: pJ6omega (plasmid)
Promoters: Promoter rat beta-actin
Construction: pBR322, SV40, rat beta-actin
Construct size (kb): 3.5
Features: marker(s): ampR
promoter: rat beta-actin
replicon: pMB1
terminator: SV40
expression vector
shuttle vector
Restriction digests of the clone give the following sizes (kb): EcoRI--3.5; HindIII--3.5; BamHI--3.5; SmaI--3.5; PvuI--2.15, 1.35.
The actin promoter has been shown to be expressed efficiently in undifferentiated cells in which transcription from other promoters (SV40 early, Mo MuLV LTR, MMTV LTR) is severely repressed.
A mammalian expression vector for expression of a sequence cloned into the polylinker site under the control of the rat beta actin promoter.
Constructed from pBR322 (2066-4286, bla, ori), SV40 (4038/4648, t antigen IVS), SV40 (2461-2704, T antigen polyadenylation site), and the rat beta-actin promoter (-340 to +10).
Media ATCC® Medium 1315: LB Medium (ATCC medium 1065) with 100 mg/L ampicillin
Growth Conditions
Temperature: 37.0°C

Morgenstern JP, Land H. A series of mammalian expression vectors and characterisation of their expression of a reporter gene in stably and transiently transfected cells. Nucleic Acids Res. 18: 1068, 1990. PubMed: 2156225

Tsutsumi M, et al. Cloning and functional expression of a mouse gonadotropin-releasing hormone receptor. Mol. Endocrinol. 6: 1163-1169, 1992. PubMed: 1324422

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