Size (kb): 3.7000000476837160
Vector: pJ4omega (plasmid)
Promoters: Promoter Mo-MuLV LTR
Construction: pBR322, SV40 Mo-MuLV
Construct size (kb): 3.700000047683716
Features: marker(s): ampR
promoter: Mo-MuLV LTR
enhancer: Mo-MuLV LTR
Restriction digests of the clone give the following sizes (kb): EcoRI--3.7; HindIII--3.7; BamHI--3.7; SmaI--3.7; PvuI--2.2, 1.5.
A mammalian expression vector for expression of a sequence cloned into the polylinker site under the control of the Mo-MuLV LTR promoter.
Constructed from pBR322 (2066-4286, bla, ori), SV40 (4038/4648, t antigen IVS), SV40 (2461-2704, T antigen polyadenylation site), and Mo-MuLV LTR.
Morgenstern JP, Land H. A series of mammalian expression vectors and characterisation of their expression of a reporter gene in stably and transiently transfected cells. Nucleic Acids Res. 18: 1068, 1990. PubMed: 2156225
Hartmut Land, personal communication