pJ4omega (ATCC® 37720)

Applications: expression vectorshuttle vector  /  Depositors: H Land

Designations pJ4omega
Permits and Restrictions

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Depositors H Land
Biosafety Level 1
Vector Information
Size (kb): 3.7000000476837160
Vector: pJ4omega (plasmid)
Promoters: Promoter Mo-MuLV LTR
Construction: pBR322, SV40 Mo-MuLV
Construct size (kb): 3.700000047683716
Features: marker(s): ampR
promoter: Mo-MuLV LTR
replicon: pMB1
terminator: SV40
enhancer: Mo-MuLV LTR
expression vector
shuttle vector
Restriction digests of the clone give the following sizes (kb): EcoRI--3.7; HindIII--3.7; BamHI--3.7; SmaI--3.7; PvuI--2.2, 1.5.
A mammalian expression vector for expression of a sequence cloned into the polylinker site under the control of the Mo-MuLV LTR promoter.
Constructed from pBR322 (2066-4286, bla, ori), SV40 (4038/4648, t antigen IVS), SV40 (2461-2704, T antigen polyadenylation site), and Mo-MuLV LTR.
Media ATCC® Medium 1315: LB Medium (ATCC medium 1065) with 100 mg/L ampicillin
Growth Conditions
Temperature: 37.0°C

Morgenstern JP, Land H. A series of mammalian expression vectors and characterisation of their expression of a reporter gene in stably and transiently transfected cells. Nucleic Acids Res. 18: 1068, 1990. PubMed: 2156225

Hartmut Land, personal communication

Shipped freeze-dried